Main Title |
Timing of Hamster Sperm Nuclear Decondensation and Male Pronucleus Formation Is Related to Sperm Nuclear Disulfide Bond Content. |
Author |
Perreault, S. D. ;
Naish, S. J. ;
Zirkin, B. R. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Johns Hopkins Univ., Baltimore, MD. Dept. of Population Dynamics. |
Year Published |
1987 |
Report Number |
EPA/600/J-87/052; |
Stock Number |
PB87-213245 |
Additional Subjects |
Germ cells ;
Cell nucleus ;
Chemical bonds ;
Hamsters ;
Time measurement ;
Reproduction(Biology) ;
Laboratory animals ;
Cytology ;
Reprints ;
Disulfides ;
Oocytes ;
Epididymis
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB87-213245 |
Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. |
|
06/21/1988 |
|
Collation |
9p |
Abstract |
The relationship between the timing of both sperm nuclear decondensation and male pronucleus formation in the oocyte and the relative level of disulfide bonds within the sperm nucleus was evaluated. Four types of hamster sperm nuclei, in which the extent of S-S bonding differed, were microinjected into hamster oocytes, and the time course of sperm nuclear decondensation and male pronucleus formation was charted. Regardless of when decondensation occurred, formation of the male pronucleus never preceded that of the female pronucleus (which occurred 1.25-1.5 hours after microinjection). However, sperm nuclei with few S-S bonds were more likely than S-S rich nuclei to transform into male pronuclei in synchrony with the formation of the female pronucleus. |