Record Display for the EPA National Library Catalog

RECORD NUMBER: 27 OF 37

Main Title Method for Recovering Viruses from River Water Solids.
Author Berg, Gerald ; Dahling, Daniel R. ;
CORP Author Environmental Monitoring and Support Lab.-Cincinnati, OH. Biological Methods Branch.
Year Published 1980
Report Number EPA-600/J-80-157 ;EMSL-191;
Stock Number PB81-126476
Additional Subjects Viruses ; Polioviruses ; Recovery ; Isolation ; Rivers ; pH ; Cell cultures ; Water ; Reprints ; Water pollution control
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB81-126476 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6p
Abstract
Small numbers of virions (poliovirus 1) that had been adsorbed to river water solids were eluted by mixing the solids for 30 minutes with a 10% solution of beef extract that contained sufficient Na2HPO4 to bring the molarity of the salt to 0.05 and sufficient citric acid to bring the pH to 7. The virions were recovered by inoculating the beef extract onto cell cultures. With this method, 39 to 63% of the poliovirions that had been adsorbed onto the river water solids were recovered.