Record Display for the EPA National Library Catalog


Main Title Light and Electron Microscopic Comparisons of Normal Hepatocytes and Neoplastic Hepatocytes of Well-Differentiated Hepotocellular Carcinomas in a Teleost Fish.
Author Couch, J. A. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL.
Publisher 24 Jun 93
Year Published 1993
Report Number EPA/600/J-94/011;
Stock Number PB94-140696
Additional Subjects Experimental hepatoma ; Fishes ; Liver ; Cell differentiation ; Cells(Biology) ; Comparison ; Microscopy ; Electron microscopy ; Diethylnitrosamine ; Reprints ; Cyprinodon variegatus
Library Call Number Additional Info Location Last
NTIS  PB94-140696 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 16p
Well-differentiated hepatocellular carcinomas (HCC's) induced in the sheepshead minnow Cyprinodon variegatus with N-nitrosodiethylamine permitted light microscopical and ultrastructural comparisons of normal hepatocytes and adjacent HCC cells. Normal hepatocytes contained typical organelles with their intracellular distribution similar to that described for other teleosts. These cells revealed a strong compartmentalization of organelles consisting of restricted perinuclear cytoplasm containing rough endoplasmic reticulum (RER) and mitochondria; most of the rest of the cell's cytoplasm volume was filled with beta-glycogen particles. Adjacent HCC cells at the border of the neoplasm interdigitated (invaded) between normal hepatocytes and replaced normal hepatocytes close to the HCC edge and adjacent to the bile canaliculi. Within the HCC, cells had little intercellular space between them and atypical bile canaliculi were found occasionally between adjacent hepatocytes. The HCC cells appeared to differ mainly in a quantitative, rather than in a qualitative fashion, and in the distribution of organelles from normal hepatocytes. Mitochondrial intermembrane myelin bodies (MMB's) were found frequently in the HCC cells but less frequently in the normal hepatocytes. The possible significance of the MMB's and intermediate, edge cells is discussed, as well as the need to better characterize features of well-differentiated hepatic neoplasms in order to assure their proper inclusion in neoplasm incidence/prevalence data in fish carcinogenesis assays and field studies.