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Main Title Flow Cytometric Analysis of the Cellular Toxicity Tributyltin.
Author Zucker, R. M. ; Elstein, K. H. ; Easterling, R. E. ; Massaro., E. J. ;
CORP Author Harry G. Armstrong Aerospace Medical Research Lab., Wright-Patterson AFB, OH. ;NSI Technology Services Corp., Dayton, OH. ;Naval Medical Research Inst., Bethesda, MD. ;Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher Nov 87
Year Published 1987
Report Number EPA/600/D-90/027;
Stock Number PB90-246117
Additional Subjects Proceedings ; Toxicology ; Fluorescence ; Deoxyribonucleic acids ; Tributyltin ; Cell survival ; Flow cytometry ; Cell membrane ; Cultured cells ; Membrane potential ; Cell differentiation ; Dose-response relationships
Library Call Number Additional Info Location Last
NTIS  PB90-246117 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 24p
Flow cytometric and light/fluorescence microscopic analyses indicate that tributyltin (TBT) alters the plasma membrane/cytoplasm complex of the murine erythroleukemic cell (MELC) in a dose dependent and time-dependent manner. The flow cytometric parameter axial light loss, a measure of cell volume, decreases in cells exposed to 5 micro M TBT relative to control cells or cells exposed to 50 micro M TBT. The flow cytometric parameter 90 deg light scatter, a function of refractive index and a measure of protein content, increases as a function of TBT concentration above 0.5 micro M, but less than 50 micro M DNA distribution across the cell cycle cannot be resolved adequately by flow cytometry. Relative to logarithmically growing cells, MELC in the stationary phase of the growth cycle and butyric acid-differentiated cells exhibit decreased plasma membrane permeability resulting in increased carboxyfluorescein (CF) retention derived from the intracellular hydrolysis of carboxyfluorescein diacetate (CFDA). Similarly, cells exposed to TBT concentrations below 50 micro M exhibit increased cellular CF retention. Viability in terms of CFDA hydrolysis/CF retention and propidium iodide (PI) exclusion is not decreased by exposure to TBT concentrations below 1 micro M. At doses between 5 and 50 micro M, however, cells exhibit both CF and PI fluorescence simultaneously and are programmed for death.