Record Display for the EPA National Library Catalog


Main Title Bacterial degredation of dibenzo-p-dioxin and chlorinated dibenzo-p-dioxins /
Author Klecka, Gary Michael. ; Klecka, G. M. ; Gibson, D. T.
Other Authors
Author Title of a Work
Gibson, D. T.
CORP Author Texas Univ. at Austin. Dept. of Microbiology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher U.S. Environmental Protection Agency, Environmental Research Laboratory,
Year Published 1981
Report Number EPA/600/4-81/016; EPA-R-804525
Stock Number PB81-171639
Subjects Chlorodibenzodioxins ; Organochlorine compounds--Biodegradation ; Catechol
Additional Subjects Biodeterioration ; Oxidation ; Oxygen heterocyclic compounds ; Chlorine aromatic compounds ; Pseudomonas ; Bacteria ; Inhibitors ; Growth regulators ; Dibenzodioxins ; Dibenzofurans ; Toxic substances ; Dioxins ; Beijerinickia ; Biodegradation ; Metabolites
Library Call Number Additional Info Location Last
NTIS  PB81-171639 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 74 pages : illustrations ; 29 cm
This report sought to determine the initial oxidative pathways for dibenzodioxin and dibenzofuran compounds by bacteria in aquatic systems. Bacterial cultures were isolated and oxidized dibenzo-p-dioxin and mono-chlorinated dibenzo-p-dioxins; however, di-chlorinated dibenzo-p-dioxins, were not metabolized by either strain. The metabolite, 1,2-dihydrodibenzo-p-dioxin, was a potent inhibitor of further oxidation. Pseudomonas sp. N.C.I.B. 9816, strain II, when grown on salicylate in the presence of dibenzo-p-dioxin, accumulated cis-1,2-dihydroxy-1,2-dihydrodibenzo-p-dioxin and 2-hydroxydibenzo-p-dioxin in the culture medium. Crude cell extracts prepared from the parental strain grown with naphthalene oxidized cis-1,2-dihydroxy-1,2-dihyrodibenzo-p-dioxin under both aerobic and anerobic conditions to 1,2-dihydroxydibenzo-p-dioxin. Further degradation of this metabolite was not detected. Whole cells of the parent strain of Beijerinckia, grown with succinate and biphenyl, oxidized dibenzo-p-dioxin and several chlorinated dioxins. The rate of oxidation of the chlorinated dibenzo-p-dioxins decreased with an increasing degree of chlorine substitution. A mutant strain (B8/36) of Beijerinckia oxidized dibenzo-p-dioxin to cis-1,2-dihydroxy-1,2-dihydrodibenzo-p-dioxin.
"March 1981." Includes bibliographical references. Microfiche.