Record Display for the EPA National Library Catalog


Main Title Stable Carbon Isotope Analysis of Nucleic Acids to Trace Sources of Dissolved Substrates Used by Estuarine Bacteria.
Author Coffin, R. B. ; Velinsky, D. J. ; Devereux, R. ; Price, W. A. ; Cifuentes, L. A. ;
CORP Author Technical Resources, Inc., Gulf Breeze, FL. ;Carnegie Institution of Washington, DC. Geophysical Lab. ;Illinois Univ. at Urbana-Champaign. Dept. of Veterinary Pathobiology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/388;
Stock Number PB91-164012
Additional Subjects Aquatic microbiology ; Tracer studies ; Organic matter ; Nucleic acids ; Isotopic labeling ; Estuaries ; Carbon isotopes ; Substrates ; Aquatic ecosystems ; Bioassay ; Deoxyribonucleic acids ; Mass spectroscopy ; Ribonucleic acids ; Extraction ; Microorganisms ; Bacteria ; Water pollution detection ; Environmental transport ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-164012 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The natural abundance of stable carbon isotopes measured in bacterial nucleic acids that were extracted from estuarine bacterial concentrates were used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of P. aeruginosa and nucleic acids extracted from cultures resembled the carbon source on which bacteria were grown. The carbon isotope discrimination (delta) between substrate and bacterial cultures averages +2.3 + or - 0.6% (n=13). Generally the lack of isotope discrimination between bacteria and nucleic acids that was noted in the laboratory was observed in the field. Exceptions to this comparison were due to changes in bacterial substrate sources as a result of the incubation experiments that were used to obtain bacteria for isotope analysis. The authors results from work in the field and laboratory indicate that the approach is useful for tracing sources of and describing the bacterial role in cycling of dissolved organic matter in aquatic environments.