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Main Title Cho/hgprt Test on Cumene with Cover Letter.
CORP Author Gulf Oil Exploration and Production Co., Oklahoma City, OK.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Year Published 1985
Report Number 878216011
Stock Number OTS0206775
Additional Subjects Toxicology ; Health effects ; Cumene (98-82-8) ; Genotoxicity ; Gene Mutations ; Mammals ; Hamsters ; In Vitro ; Toxic substances ; Laboratory animals ; CAS No 98-82-8
Library Call Number Additional Info Location Last
NTIS  OTS0206775 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 29p
The ability of cumene to induce mutations at the gene locus coding for hypoxanthine-guanine phosphoribosyl transferase in Chinese hamster ovary cells (CHO/HGPRT Assay) was evaluated in the presence and absence of metabolic activation from Aroclor-induced rat liver S9 fraction. Based on preliminary cytotoxicity tests, nonactivated cultures were treated with cumene at concentrations of 8, 16, 32, 64, and 128 mcg/ml producing cell survivals relative to the vehicle control (Pluronic F127) of 122.9, 92.8, 104.7, 124.6, and 89.2% respectively. Activated cultures treated with cumene concentrations of 64, 128, 150, and 175 mcg/ml produced relative cell survivals ranging from 91.0 - 7.6%. A significant mutagenic response was not observed in either activated or nonactivated trials.