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RECORD NUMBER: 4 OF 11

OLS Field Name OLS Field Data
Main Title Four Final Mutagenicity Reports Regarding Phthalate Esters.
CORP Author Hazleton Biotechnologies, Kensington, MD.; Chemical Manufacturers Association, Washington, DC.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Year Published 2000
Report Number 40-8626225
Stock Number OTS0510527
Additional Subjects Toxicology ; Health effects ; Phthalate Esters ; Genotoxicity ; Gene Mutations ; Mammals ; Mice ; In Vitro ; Toxic substances ; Laboratory animals ; CAS No 84-74-2 ; CAS No 85-68-7 ; CAS No 131-11-3 ; CAS No 68515-51-5
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NTIS  OTS0510527 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/10/2010
Collation 108p
Abstract
The ability of butyl benzyl phthalate to induce specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells (Mouse Lymphoma Mutagenicity Assay) was evaluated in the presence and absence of Aroclor-induced rat liver S-9 metabolic activation. Based on preliminary toxicity tests, 16 nonactivated cultures treated from 6.25nl/ml to 40.0nl/ml were cloned, producing a range of 83 - 2.5% relative growth. Twenty S-9 activated cultures treated from 200 to 1400nl/ml were cloned, producing a range of 65.7 - 1.0% relative growth. None of the cultures produced mutant frequencies significantly greater than the solvent control (acetone). Di-(n-hexyl, n-octyl, n-decyl) phthalate (DHODP), dimethyl phthalate and di-n-butyl phthalate were also evaluated in the Mouse Lymphoma Mutagenicity Assay in the same manner as above. Based on preliminary toxicity tests, 10 nonactivated cultures treated from 2500 nl DHODP/ml to 8000 nl/ml were cloned, producing a relative growth range of 43.1 - 2.4%. Ten S-9 activated cultures treated from 150 nl DHODP/ml to 400 nl/ml were cloned, producing a relative growth range of 82.2 - 9.2&. With the exception of the 150 nl/ml activated culture, all cultures with or withour metabolic activation produced mutant frequencies greater than the solvent control. In the next study, based on preliminary toxicity tests, 10 nonactivated cultures treated with 15 nl dimethyl phthalate/ml to 40 nl/ml were cloned, producing a relative growth range of 83 - 11.3%. Ten S-9 activated cultures were then treated with 600 nl dimethyl phthalate/ml to 1400 nl/ml, producing a relative growth range of 65.7 - 6.2%. None of the dimethyl phthalate cultures produced mutant frequencies significantly greater than the solvent control. Also based on preliminary toxicity tests, 4 nonactivated cultures treated with di-n-butyl phthalate at concentrations of 25 nl/ml and 50 nl/ml produced relative growth ranges of 79.2 - 48.7%. Six S-9 activated cultures were then treated with 25 - 75 nl/ml, producing relative growth ranges of 91.1 - 6.9%. All of the S-9 activated di-n-butyl phthalate cultures produced mutant frequencies greater than the solvent controls.