Record Display for the EPA National Library Catalog


Main Title Mutagenicity Evaluation of Hexachlorocyclopentadiene in the Mouse Lymphoma Forward Mutation Assay (final Report) Project Number 20839 (February 1978).
CORP Author Velsicol Chemical Corp., Chicago, IL.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Year Published 2000
Report Number 40-7849061
Stock Number OTS0512869
Additional Subjects Toxicology ; Health effects ; Hexachlorocyclopentadiene ; Genotoxicity ; Gene Mutations ; Mammals ; Mice ; In Vitro ; Toxic substances ; Laboratory animals ; CAS No 77-47-4
Library Call Number Additional Info Location Last
NTIS  OTS0512869 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
The ability of hexachlorocyclopentadiene to induce specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells (Mouse Lymphoma Forward Mutation Assay) was evaluated in the presence and absence of mouse liver S9 metabolic activation (inducer not reported). Based on preliminary toxicity tests, nonactivated cultures were treated with hexachlorocyclopentadiene at concentrations of 0.0000025, 0.0000050, 0.0000100, 0.0000200, and 0.0000400 ul/ml producing a range of 0.3 - 82.0 % growth relative to the solvent (DMSO) control. Additionally, activated cultures were treated with concentrations of 0.00002, 0.00004, 0.00008, 0.00016, 0.00032, 0.00040, 0.00048, 0.00056, 0.00064, and 0.00125 ul/ml producing growth relative to the solvent control ranging from 2.2 - 115.8%. Hexachlorocyclopentadiene did not induce a positive mutagenic response either with or without metabolic activation.