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RECORD NUMBER: 4 OF 5

OLS Field Name OLS Field Data
Main Title Mutagenicity of Dimethyl Phthalte in a Mouse Lymphoma Mutation Assay (Final Report).
CORP Author Hazleton Biotechnologies, Kensington, MD.; Chemical Manufacturers Association, Washington, DC.; Environmental Protection Agency, Washington, DC. Office of Toxic Substances.
Year Published 1986
Report Number 40-8626244
Stock Number OTS0510741
Additional Subjects Toxicology ; Health effects ; Bis(2-ethylhexyl)terephthalate ; Genotoxicity ; Gene Mutations ; Mammals ; Mice ; In Vitro ; Toxic substances ; Laboratory animals ; CAS No 131-11-3
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NTIS  OTS0510741 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/10/2010
Collation 26p
Abstract
The ability of dimethyl phthalate to induce specific locus mutations at the TK locus in cultured L5178Y mouse lymphoma cells (Mouse Lymphoma Mutagenicity Assay) was evaluated in the presence and absence of Aroclor-induced rat liver S9 metabolic activation. Dimethyl phthalate, diluted with acetone, was incompletely soluble at concentrations above 700 nl/ml without activation and above 500 nl/ml in the presence of metabolic activation. Non- activated cultures treated in duplicate with 200, 300, 400, 500, and 600 nl/ml produced a range of 101.7 to 52.5% relative growth. Since highly toxic treatments were not achieved, a second trialwith 8 non-activated cultures were treated in duplicate with 400, 500, 650, and 700 nl/ml, producing a range of 98.1 to 26.9% relative growth. S9 activated cultures treated in duplicate with 100, 200, 400, 500, and 600 nl/ml produced a range of 80.3 to 14.0% relative growth. Non-activated cultures at 500 nl/ml and 650 nl/ml produced mutant frequencies significantly greater than the solvent control (acetone); however, a dose-dependent response was not observed. All of the activated cultures produced mutant frequencies significantly greater than the solvent control.