Record Display for the EPA National Library Catalog
RECORD NUMBER: 76 OF 100Main Title | Numerical Methods for the Life Scientist Binding and Enzyme Kinetics Calculated with GNU Octave and MATLAB / [electronic resource] : | ||||
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Type | EBOOK | ||||
Author | Prinz, Heino. | ||||
Publisher | Springer Berlin Heidelberg : Imprint: Springer, | ||||
Year Published | 2011 | ||||
Call Number | QH324.2-324.25 | ||||
ISBN | 9783642208201 | ||||
Subjects | Life sciences ; Bioinformatics | ||||
Internet Access |
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Collation | IX, 149p. 61 illus., 52 illus. in color. online resource. | ||||
Notes | Due to license restrictions, this resource is available to EPA employees and authorized contractors only |
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Contents Notes | Preparing the computer -- Binding equilibria -- Binding kinetics -- Least squares fit to experimental data -- Multi-parameter fits -- Comparing the two different approaches. . Enzyme kinetics, binding kinetics and pharmacological dose-response curves are currently analyzed by a few standard methods. Some of these, like Michaelis-Menten enzyme kinetics, use plausible approximations, others, like Hill equations for dose-response curves, are outdated. Calculating realistic reaction schemes requires numerical mathematical routines which usually are not covered in the curricula of life science. This textbook will give a step-by-step introduction to numerical solutions of non-linear and differential equations. It will be accompanied with a set of programs to calculate any reaction scheme on any personal computer. Typical examples from analytical biochemistry and pharmacology can be used as versatile templates. When a reaction scheme is applied for data fitting, the resulting parameters may not be unique. Correlation of parameters will be discussed and simplification strategies will be offered. |