Record Display for the EPA National Library Catalog


Main Title New approaches to the preservation of contaminants in water samples /
Author Saxena, J.
Other Authors
Author Title of a Work
Nies, E.
CORP Author Syracuse Research Corp., NY. Center for Chemical Hazard Assessment.;Environmental Monitoring and Support Lab.-Cincinnati, OH.
Publisher Environmental Protection Agency, Office of Research and Development, Environmental Monitoring and Support Laboratory,
Year Published 1979
Report Number EPA 600-4-79-007; EPA-R-804609010
Stock Number PB-293 013
OCLC Number 04787480
ISBN pbk.
Subjects Water--Analysis
Additional Subjects Samples ; Preservatives ; Sewage ; Water pollution ; Chemical analysis ; Storage ; Cargo transport ; Nitrogen ; Inorganic phosphates ; Biochemical oxygen demand ; Oils ; Greases ; pH ; Antibiotics ; Enzymes ; Biocides ; Substitutes ; Stability ; Antimicrobial agents ; Water pollution sampling
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD  EPA 600-4-79-007 c.1 Headquarters Library/Washington,DC 04/02/2014
ELBD ARCHIVE EPA 600-4-79-007 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD  EPA 600-4-79-007 AWBERC Library/Cincinnati,OH 11/14/2023
NTIS  PB-293 013 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation xi, 46 pages : illustrations, figures, tables ; 28 cm
The potential of antibiotics, chemical biocides and lytic enzymes in preserving nutrients, biological oxygen demand and oil and grease in water and sewage effluents was studied. Preliminary studies concerning the effect of drugs on cell growth and oxygen utilization in samples stored at room temperature led to the selection of chlorhexidine, vantocil and many combinations each containing three antibiotics from among polymyxin B, neomycin, erythromycin, streptomycin and chloramphenicol. The effective concentration range was approximately equal to 50 micrograms/ml each antibiotic for clean waters and as high as 200 micrograms/ml each antibiotic for heavily polluted water (e.g. primary effluents). Chlorhexidine and vantocil IB stabilized nitrate and nitrite in fresh waters and relatively clean secondary effluents only. Presence of antibiotics caused interference in determination of orthophosphate. The antimicrobial agents tested interfered with BOD determination by causing inhibition of oxygen depletion and therefore were of no value for preservation of this parameter. Efforts to selectively remove and/or inactivate the drug before BOD determination were unsuccessful. Oil and grease levels were stabilized by antibiotics for up to two weeks in relatively clean waters only. The results demonstrate that antibiotics offer a viable alternative to conventional methods for preservation of some constituents and parameters in fresh water samples but not in sewage effluents.
U.S. Environmental Protection Agency Grant No. Contract Number: R 804609010. "January 1979." Includes bibliographical references (pages 41-45).