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RECORD NUMBER: 668 OF 1236

Main Title Measurement of Methanotroph and Methanogen Signature Phosopholipids for Use in Assessment of Biomass and Community Structure in Model Systems.
Author Nichols, P. D. ; Mancuso, C. A. ; White, D. C. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science. ;Commonwealth Scientific and Industrial Research Organization, Hobart (Australia). Div. of Oceanography. ;Tennessee Univ., Knoxville. Inst. for Applied Microbiology.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Year Published 1987
Report Number EPA-R-812504; EPA/600/J-87/359;
Stock Number PB88-219811
Additional Subjects Soils ; Biomass ; Spirillacea ; Natural gas ; Biochemistry ; Fatty acids ; Lipids ; Digestion(Biology) ; Methane ; Profiles ; Oxidation reduction reactions ; Graphs(Charts) ; Reprints ; Methanogea
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NTIS  PB88-219811 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Abstract
Methanotrophic biomass and community structure were assessed for a soil column enriched with natural gas. An increase in microbial biomass, based on phospholipid ester-linked fatty acids (PLFA), was apparent for the natural gas-enriched column relative to a control column and untreated surface soil. Following GC-MS analyses of the derivatized monounsaturated fatty acids, the major component (22 percent of the PLFA) of the natural gas-enriched column was identified as 18:1 delta 10C. The relatively novel fatty acid has only been previously reported as a major component in methanotrophs. Its presence in the soil, together with other supportive evidence, implies that the microbial metabolic group makes a large contribution to the column flora. Other microbial groups were also recognized and differences compared between the soils analyzed. (Copyright (c) 1987 Pergamon Journals Ltd.)