||Benzoate-Dependent Induction from the OP2 Operator-Promoter Region of the TOL pWWO in the Absence of Known Plasmid Regulatory Genes (Journal Version).
Cuskey, S. M. ;
Sprenkle, A. B. ;
||Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL.
Enzyme induction ;
Microbial genetics ;
Gene expression regulation ;
Molecular cloning ;
Genetic transformation ;
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The OP2 (Pm) operator-promoter region of the TOL plasmid, pWWO, is located immediately upstream from the 'lower' pathway operon responsible for the catabolism of benzoate and related aromatic acids. Induction of the operon in the presence of aromatic acid inducers, requires the xylS gene product. The authors cloned a 401 base pair region containing the OP2 region upstream from the promoter less xylE (catechol 2,3-dioxygenase) gene and measured induction in the presence of a variety of aromatic inducers in a xylS free background. Approximately 30-fold induction was seen when transformed cells of Pseudomonas putida strain PRS2000 were grown on lactate in the presence of benzoate. Slight (4-fold) induction occurred when m-toluic acid was included in the growth medium.