Record Display for the EPA National Library Catalog
RECORD NUMBER: 42 OF 92| Main Title | Metabolism of Benzo(a)Pyrene and Persistence of DNA Adducts in the Brown Bullhead 'Ictalurus nebulosus'. | |||||||||||
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| Author | Sikka, H. C. ; Steward, A. R. ; Kandaswami, C. ; Rutkowski, J. P. ; Zaleski, J. ; | |||||||||||
| CORP Author | State Univ. of New York Coll. at Buffalo. Div. of Environmental Toxicology and Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.;Kentucky Univ., Lexington. Medical Center.;Rutgers - The State Univ., Piscataway, NJ. Dept. of Pharmacology and Toxicology. | |||||||||||
| Publisher | c1991 | |||||||||||
| Year Published | 1991 | |||||||||||
| Report Number | EPA-R-813799; EPA/600/J-92/200; | |||||||||||
| Stock Number | PB92-189067 | |||||||||||
| Additional Subjects | Benzo(a)pyrene ; Metabolism ; DNA adducts ; Toxicology ; Liver microsomes ; Thin layer chromatography ; Stereochemistry ; Glutathione ; Glucuronates ; Reprints ; Brown bullhead ; Ictalurus nebulosus ; Benzo(a)pyrene dihydrodiol | |||||||||||
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| Collation | 6p | |||||||||||
| Abstract | The in vitro metabolism of ((3)H)benzo(a)pyrene (BP) and ((14)C)benzo(a)pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. Compared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of BP-7,8-diol (predominantly the (-) enantiomer) and smaller amounts of BP-4,5-diol. BP phase I metabolites were efficiently converted by freshly isolated bullhead hepatocytes to conjugates, predominantly glucuronides. BP-7,8-diol was metabolized by hepatocytes 4-fold more rapidly than was BP and was converted to approximately equal amounts of glucuronides, glutathione conjugates and sulfates. BP-DNA adducts formed in bullhead liver with a lag time of several days and maximum adduct formation at 25-30 days. The major adduct was anti-BPDE-deoxyguanosine. (Copyright (c) 1991 Pergamon Press.) |