Record Display for the EPA National Library Catalog


Main Title Interactions between 'Bacillus thuringiensis subsp. 'israelensis' and Fathead Minnows, 'Pimephales promelas' Rafinesque, under Laboratory Conditions.
Author Snarski, V. M. ;
CORP Author Environmental Research Lab.-Duluth, MN.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/253;
Stock Number PB91-144923
Additional Subjects Biological pest control ; Bacillus thuringiensis ; Water microbiology ; Feces ; Bacteriological techniques ; Water pollution effects(Animals) ; Bacterial spores ; Colony-forming units ; Reprints ; Pimephales promelas
Library Call Number Additional Info Location Last
NTIS  PB91-144923 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Interactions between Bacillus thuringiensis subsp. israelensis and fathead minnows, Pimephales promelas, were studied in laboratory exposures to two commercial formulations, Vectobac-G and Mosquito Attack. Mortality among fatheads exposed to 2.0 x 10 to the sixth power to 6.5 x 10 to the sixth power CFU/ml with both formulations was attributed to severe dissolved oxygen depletion due to formulation ingredients rather than to direct toxicity from the parasporal crystal. No adverse effects were observed at 6.4 x 10 to the fifth power CFU/ml and below. Fathead minnows rapidly accumulated high numbers of spores with 1 h of exposure to 2.2 x 10 to the fifth power CFU of Mosquito Attack per ml, producing whole-body counts of 4.0 x 10 to the sixth power CFU per fish. Comparison of counts on gastrointestinal tract samples and whole-body samples and high numbers of spores in feces indicated that ingestion was the major route of exposure. B. thuringiensis subsp. israelensis spore counts decreased rapidly after transfer of fish to clean water, with a drop of over 3 orders of magnitude in 1 day. Spores were rarely detected in fish after 8 days but were detectable in feces for over 2 weeks. These findings suggest that fish could influence the dissemination of B. thuringiensis subsp. israelensis, and possibly other microbial agents, in the aquatic environment. (Copyright (c) 1990, American Society for Microbiology.)