Record Display for the EPA National Library Catalog


Main Title Genetic engineering of enhanced microbial nitrification /
Author Carsiotis, Michael. ; Khanna, S.
Other Authors
Author Title of a Work
Khanna, Sunil.
CORP Author Cincinnati Univ., OH. Dept. of Microbiological and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher U.S. Environmental Protection Agency, Risk Reduction Engineering Laboratory,
Year Published 1989
Report Number EPA/600-M-89-011; EPA-R-810888
Stock Number PB89-208334
OCLC Number 21115522
Subjects Nitrification ; Genetic engineering
Additional Subjects Nitrification ; Nitrobacter ; Bacterial proteins ; Genetic engineering ; Resistance plasmids ; Nitrobacter hamburgenesis ; Genetic vectors ; Transfection ; Waste water treatment
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHAM  EPA/600/M-89/011 Region 1 Library/Boston,MA 05/25/2016
EJBD  EPA 600-M-89-011 c.1 Headquarters Library/Washington,DC 11/01/2013
ELBD ARCHIVE EPA 600-M-89-011 In Binder Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD RPS EPA 600-M-89-011 repository copy AWBERC Library/Cincinnati,OH 10/17/2014
NTIS  PB89-208334 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 6, [1] pages : illustrations ; 28 cm.
Experiments were conducted to introduce genetic information in the form of antibiotic or mercuric ion resistance genes into Nitrobacter hamburgenesis strain X14. The resistance genes were either stable components of broad host range plasmids or transposable genes on plasmids presumably unable to replicate in strain X14. Four methods for plasmid transformation as well as conjugation with various donor strains of Escherichia coli failed. The leuB gene containing DNA was restriction-mapped and the 1.3 kilobase pair gene was subcloned into a vector suitable for use in DNA sequencing. To date, a tentative sequence comprising about 1300 bases has been obtained. Although the primary goal of developing a procedure for introducing genetic material into a nitrifying organism has not yet been achieved, the results achieved have produced useful information on the genomic organization of Nitrobacter as well as a plasmid-borne library of genes from that organism. Future experiments can be made with this library in order to provide additional basic information on Nitrobacter's genome.
Caption title. Shipping list no.: 89-379-P. "June 1989." Includes bibliographical references (pages 6-7). "EPA/600-M-89-011."