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Main Title Role of Na(+) in Transport of Hg(2+) and Induction of the Tn21 mer Operon.
Author Selifonova, O. V. ; Barkay, T. ;
CORP Author University of West Florida, Pensacola. Center for Environmental Diagnostics and Bioremediation.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1994
Year Published 1994
Report Number EPA-R-819770; EPA/600/J-94/501;
Stock Number PB95-155206
Additional Subjects Mercury ; Aquatic microbiology ; Genes ; Sodium ; Induction ; Transport properties ; Ion ion interactions ; Escherichia coli ; Uptake ; Gradients ; Reprints ; Bacterial genes ; TN21 mer operon
Library Call Number Additional Info Location Last
NTIS  PB95-155206 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
The effects of sodium ions on the uptake of Hg(2+) and induction of the TN21 mer operon were studied using Escherichia coli (E. coli) HMS174 harboring the reporter plasmids pRB28 and pOS14. Plasmid pRB28 carried merRT' and pOS14 carried merRTPC of the mer operon, both cloned upstream of a promoterless luciferase gene cassette in pUDC615. The bioluminescent response to 1 microM Hg(2+) was significantly inhibited in E. coli HMS174(pRB28) in minimal medium supplemented with sodium ions at 10 to 140 mM. Sodium ions appear to facilitate mercury transport. The authors propose that sodium-coupled transport of mercuric ions can be one of the mechanisms for mercury uptake by E. coli and that the Na(1+) gradient may energize the transport of Hg(2+).