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RECORD NUMBER: 13 OF 15

Main Title Screening Complex Hazardous Wastes for Mutagenic Activity Using a Modified Version of the TLC/Salmonella Assay.
Author Houk, V. S. ; Claxton, L. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;North Carolina Univ. at Chapel Hill. School of Public Health.
Year Published 1986
Report Number EPA/600/J-86/024;
Stock Number PB86-187234
Additional Subjects Hazardous materials ; Wastes ; Bacteria ; Toxicology ; Bioassay ; Reprints ; Salmonella ; Mutagenesis
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB86-187234 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Abstract
Ten complex hazardous wastes were tested for mutagenic activity using a modified version of the TLC/Ames assay developed by Bjorseth et al. (1982). This fractionation/bioassay scheme couples thin layer chromatography (TLC) with the Salmonella/mammalian-microsome (Ames) assay for the detection of mutagenic constituents in complex mixtures. Crude (unadulterated) hazardous wastes and selected hazardous waste extracts were fractionated on commercially available cellulose TLC plates. Mutagenicity testing was performed in situ by applying a single overlay of minimal growth agar, tester strain TA98 or TA100, and the optional metablic activation system directly onto the developed chromatogram. A mutagenic effect was indicated either by the appearance of localized clusters of revertant colonies or by an increase in total revertant growth vis-a-vis control plates. Seven of ten hazardous wastes (including tars, emulsions, sludges, and spent acids and caustics) demonstrated mutagenic activity when tested by this method.