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Main Title Microbial Degradation of Toluene under Sulfate-Reducing Conditions and the Influence of Iron on the Process.
Author Beller, H. R. ; Grbic-Galic, D. ; Reinhard., M. ;
CORP Author Stanford Univ., CA. Dept. of Civil Engineering.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher c1992
Year Published 1992
Report Number EPA-R-815721; EPA/600/J-92/139;
Stock Number PB92-166735
Additional Subjects Land pollution control ; Microbial degradation ; Toluene ; Sulfate reducing bacteria ; Water pollution control ; Anaerobic processes ; Subsurface investigations ; Aviation fuels ; Biodeterioration ; Environmental transport ; Biocontrol ; Iron ; Oxidation ; Chemical reactions ; Storage tanks ; Biochemistry ; Aquifers ; Patuxent River ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-166735 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Toluene degradation occurred concomitantly with sulfate reduction in anaerobic microcosms inoculated with contaminated subsurface soil from an aviation fuel storage facility near the Patuxent River (Md.). Similar results were obtained from enrichment cultures in which toluene was the sole carbon source. Several lines of evidence suggest that toluene degradation was directly coupled to sulfate reduction in Patuxent River microcosms and enrichment cultures: (1) the two processes were synchronous and highly correlated, (2) the observed stoichiometric ratios of moles of sulfate consumed per mole of toluene consumed were consistent with the theoretical ratio for the oxidation of toluene to CO2 coupled with the reduction of sulfate to hydrogen sulfide, and (3) toluene degradation ceased when sulfate was depleted, and conversely, sulfate reduction ceased when toluene was depleted. Mineralization of toluene was confirmed in experiments with (ring-U-14C)toluene. The addition of millimolar concentrations of amorphous Fe(OH)3 to Patuxent River microcosms and enrichment cultures either greatly facilitated the onset of toluene degradation or accelerated the rate once degradation had begun. In iron-amended microcosms and enrichment cultures, ferric iron reduction proceeded concurrently with toluene degradation and sulfate reduction. Stoichiometric data and other observations indicate that ferric iron reduction was not directly coupled to toluene oxidation but was a secondary, presumably abiotic, reaction between ferric iron and biogenic hydrogen sulfide. (Copyright (c) 1992, American Society for Microbiology.)