| Main Title |
Preparation of Research Reagents for 2 Dengue Strains. |
| Author |
Shope., Robert E. ;
|
| CORP Author |
Yale Univ., New Haven, Conn. Yale Arbovirus Research Unit.;National Institutes of Health, Bethesda, Md.;National Inst. of Allergy and Infectious Diseases, Bethesda, Md. Research Resources Branch. |
| Year Published |
1976 |
| Report Number |
N01-AI-2-2509; NIAID/RR/F-2509-76; |
| Stock Number |
PB-257 364 |
| Additional Subjects |
Dengue virus ;
Arboviruses ;
Antibodies ;
Antigens ;
Standards ;
Mice ;
Viruses ;
Serology ;
Research ;
Reagents ;
Ascitic fluid
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB-257 364 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
14p |
| Abstract |
Four lots totalling 3,050 ml of dengue 2 mouse immune ascitic fluid; 5 lots totalling 3,365 ml of dengue 3 immune ascitic fluid; and two lots of 200 ml each of control ascitic fluids were prepared. Plaque reduction neutralization tests indicated that most lots of dengue 2 fluids had homologous neutralization about 32-fold greater than heterologous. Dengue 3 fluids were less specific, with homologous titers 4-8 fold greater than heterologous. Seed virus reagents for dengue 2 and dengue 3 viruses had titers of 9.0 log LD50/ml and 7.0 log LD50/ml respectively. Control ascitic fluids did not neutralize dengue viruses. |
