The Direct/Delayed Response Project (DDRP) has been designed and implemented by the U.S. Environmental Protection Agency to reduce uncertainties in current understanding of the effects of acidic deposition on surface waters chemistry. The response of watersheds to acidic deposition is being assessed for selected study regions using data from DDRP watershed surveys, USEPA surface water surveys, and acidic deposition data. The document describes the planning activities, field activities, and the quality assurance/quality control (QA/QC) operations associated with soil sampling in the Mid-Appalachian Region of the United States. The watersheds mapped were chosen from those studied by the USEPA National Surface Water Survey. The objective of the soil sampling was to collect samples to characterize the physical and chemical properties of soil from watersheds that were mapped by the DDRP. The activities resulted in 100 percent of the selected pedons being sampled.