As part of the U.S. Environmental Protection Agency's effort to determine the long-term effects of acidic deposition on surface water chemistry, annual runoff was estimated for about 1000 ungaged sites in the eastern U.S. using runoff contour maps. One concern in using contour maps was that a bias may be introduced in the runoff estimates due to the size of the 1000 ungaged sites relative to the size of the watersheds used in developing the maps. To determine if a bias was present the relationship between the annual runoff (expressed as depth) and the watershed area for the Northeast (NE) and Southern Blue Ridge Province (SBRP) was tested using five regional data bases. One short-term data base (1984 Water Year, n = 531), and two long-term data bases (1940-57, n = 134 and 1951-80, n = 342) was used in the NE. In the SBRP one short-term data base (1984 Water Year, n = 531) and one long-term data base (1951-80, n = 60) were used. For the NE and the SBRP, runoff was not directly correlated with watershed area using the five regional data bases. Also, runoff normalized by precipitation was not related to watershed area.