Main Title |
Embryonic Development in vitro Following Short-Duration Exposure to Heat. |
Author |
Kimmel, G. L. ;
Cuff, J. M. ;
Kimmel, C. A. ;
Heredia, D. J. ;
Tudor, N. ;
|
CORP Author |
Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment. ;Thiel Coll., Greenville, PA. Dept. of Biology. ;Food and Drug Administration, Rockville, MD. Center for Devices and Radiological Health. |
Publisher |
c1993 |
Year Published |
1993 |
Report Number |
EPA/600/J-93/186 ;OHEA-R-496; |
Stock Number |
PB93-194538 |
Additional Subjects |
Embryo development ;
Induced hyperthermia ;
Heat ;
Rats ;
Body temperature ;
In vitro analysis ;
Growth ;
Animal pregnancy ;
Exposure ;
Reprints ;
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB93-194538 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
Gestation day (GD) 10 rat embryos (10-12 somites) were exposed in vitro for 10 to 25 mins at 42 C or 43 C and evaluated 24 hrs later for alterations in growth and specific morphological parameters, using a modified Brown-Fabro scoring system that allowed evaluation of development relative to gestational age. At 42 C, crown-rump length appeared to be particularly sensitive, responding to only 10 mins exposure. A 15-min exposure resulted in decreased total protein, somite number and morphological score. At 43 C, all of the parameters measured were affected by a 10-min exposure. The response patterns demonstrated a clear temperature- and exposure duration-dependency, with a shift from a more shallow duration-response curve to a more dramatic inhibition of development as temperature increased from 42 C to 43 C. |