Record Display for the EPA National Library Catalog


Main Title Embryonic Development in vitro Following Short-Duration Exposure to Heat.
Author Kimmel, G. L. ; Cuff, J. M. ; Kimmel, C. A. ; Heredia, D. J. ; Tudor, N. ;
CORP Author Environmental Protection Agency, Washington, DC. Office of Health and Environmental Assessment. ;Thiel Coll., Greenville, PA. Dept. of Biology. ;Food and Drug Administration, Rockville, MD. Center for Devices and Radiological Health.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/186 ;OHEA-R-496;
Stock Number PB93-194538
Additional Subjects Embryo development ; Induced hyperthermia ; Heat ; Rats ; Body temperature ; In vitro analysis ; Growth ; Animal pregnancy ; Exposure ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-194538 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Gestation day (GD) 10 rat embryos (10-12 somites) were exposed in vitro for 10 to 25 mins at 42 C or 43 C and evaluated 24 hrs later for alterations in growth and specific morphological parameters, using a modified Brown-Fabro scoring system that allowed evaluation of development relative to gestational age. At 42 C, crown-rump length appeared to be particularly sensitive, responding to only 10 mins exposure. A 15-min exposure resulted in decreased total protein, somite number and morphological score. At 43 C, all of the parameters measured were affected by a 10-min exposure. The response patterns demonstrated a clear temperature- and exposure duration-dependency, with a shift from a more shallow duration-response curve to a more dramatic inhibition of development as temperature increased from 42 C to 43 C.