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Main Title Metabolism of the (18)O-Methoxy Substituent of 3-Methoxybenzoic Acid and Other Unlabeled Methoxybenzoic Acids by Anaerobic Bacteria.
Author DeWeerd, K. A. ; Saxena, A. ; Nagle, D. P. ; Suflita, J. M. ;
CORP Author Oklahoma Univ., Norman. Dept. of Botany and Microbiology.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/384;
Stock Number PB90-103037
Additional Subjects Metabolism ; Anaerobic bacteria ; Biomass ; Mass spectroscopy ; Reprints ; Methoxybenzoic acids ; Liquid column chromatography
Library Call Number Additional Info Location Last
NTIS  PB90-103037 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
O-methyl substituents of aromatic compounds can provide C1 growth substrates for facultative and strict anaerobic bacteria isolated from diverse environments. The mechanism of the bioconversion of methoxylated benzoic acids to the hydroxylated derivatives was investigated with a model substrate and cultures of one anaerobic consortium, eight strict anaerobic bacteria, and one facultative anaerobic micro-organism. A haloaromatic dehalogenating consortium, a dehalogenating isolate from that consortium, Eubacterium limosum, and a strain of Acetobacterium woodii metabolized 3-methoxybenzoic acid to 3-hydroxybenzoic stoichiometrically at rates of 1.5, 3.2, 52.4, and 36.7 nmol/min per mg of protein, respectively. A different strain of Acetobacterium and strains of Syntrophococcus, Clostridium, Desulfotomaculum, Enterobacter, and an anaerobic bacterium, strain TH-001, were unable to transform this compound. The O-demethylating ability of E. limosum was induced only with appropriate methoxylated benzoates but not with D-glucose, lactate, isoleucine, or methanol. (Copyright (c) 1988 American Society for Microbiology.)