Record Display for the EPA National Library Catalog


Main Title Development of In vivo Genotoxicity Tests in Estuarine Fish and Their Application to Aquatic Toxicology.
Author Means, J. C. ; Daniels, C. B. ; Baksi, S. M. ;
CORP Author Louisiana State Univ., Baton Rouge. Inst. for Environmental Studies. ;Johns Hopkins Univ., Baltimore, MD. School of Hygiene and Public Health.;Environmental Research Lab., Narragansett, RI.
Publisher 1988
Year Published 1988
Report Number EPA/600/J-88/463 ; ERLN-P06
Stock Number PB90-147695
Additional Subjects Toxicology ; Marine fishes ; In vivo analysis ; Cyclophosphamide ; Exposure ; Mutagens ; Kidney ; Liver ; Gastrointestinal system ; Estuaries ; Reprints ; Toxic substances ; Mutagenicity tests ; Aminoacridines ; Ethyl methanesulfonate ; N-methyl-n-nitro-n-nitroguanidine ; Dose-response relationships
Library Call Number Additional Info Location Last
NTIS  PB90-147695 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Genotoxicity assays were developed in embryonic stages of two species of fish, striped bass (Morone saxitilis) and sheepshead minnow (Cyprinodon variegatus), and in the adults of Fundulus heteroclitus using metaphase chromosome aberrations as the endpoint. Dose-dependent responses were obtained with several chemical mutagens, including 9-aminoacridine, ethylmethane sulphonate, cyclophosphamide and n-methyl-n-nitro-n-nitroguanidine, added to estuarine water at doses spanning several orders of magnitude in compound concentration. Exposures ranged from one to four days; however, two-day exposure time was found to be optimal in eggs and larvae. Tissues from the gills, kidney and intestinal tract of adult Fundulus were found to be responsive to mutagen exposure; however, the intestine gave the best responses. The results of these experiments suggest that these assays are sufficiently sensitive to be used in the field as well as in laboratory tests. (Copyright (c) 1988 Elsevier Applied Science Publishers, Ltd.)