Record Display for the EPA National Library Catalog


Main Title Identification and Quantitation of Alkylated Nucleobases by High-Performance Liquid Chromatography with UV Photodiode Array Detection.
Author Xue, W. ; Samanta, A. K. ; Carlson, R. M. ;
CORP Author Minnesota Univ.-Duluth. Dept. of Chemistry. ;Academia Sinica, Beijing (China). Research Center for Eco-Environmental Sciences.;Environmental Research Lab.-Duluth, MN.
Publisher c1990
Year Published 1990
Report Number EPA-R813144-02 ;EPA-R813943; EPA/600/J-90/252;
Stock Number PB91-144931
Additional Subjects Nucleosides ; Liquid chromatography ; Ultraviolet detectors ; Alkylation ; Molecular structure ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB91-144931 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The application of UV diode array detection in high-performance liquid chromatographic (HPLC) identification and quantification of several classes of synthetic and commercially available alkylated nucleobases is investigated. Quantitative spectral overlays of these compounds to methyl standard references from a spectral library and absorbance ratios at two maximal wavelengths are found to be useful in categorizing the solutes. They can be grouped into classes of compounds originating from a specific nucleobase and classes of analogs having different alkyl substituents (e.g., methyl, ethyl, propyl, allyl, and benzyl) at the same position of the heterocycle. At a selected wavelength for alkylated nucleobases in the same class, the detector response factors are independent of the alkyl group (+ or - 10%). This technique provides a practical means for both qualitative and quantitative analysis of product distribution of DNA base alkylation by using only readily obtainable methylated derivatives as the reference standards.