Record Display for the EPA National Library CatalogRECORD NUMBER: 4 OF 9
|Main Title||Long-Term Effects of Triethylenemelamine Exposure on Mouse Testis Cells and Sperm Chromatin Structure Assayed by Flow Cytometry.|
|Author||Evenson, D. P. ; Baer, R. K. ; Jost, L. K. ;|
|CORP Author||South Dakota State Univ., Brookings. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.|
|Report Number||EPA-R-810991; EPA/600/J-89/374;|
|Additional Subjects||Testis ; Toxicity ; Graphs(Charts) ; Mice ; Exposure ; Body weight ; Alkylating agents ; Reprints ; Spermatozoa ; Chromatin ; Flow cytometry ; Triethylenemelamine ; Mutagenicity tests ; Organ weight ; Acridine orange|
The toxic and potentially mutagenic actions of triethylenemelamine (TEM) on mouse body and testis weights, testicular cell kinetics, sperm production, sperm head morphology, and sperm chromatin structure were assessed in two experiments. The first experiment examined effects of four dose levels of TEM, assayed 1, 4, and 10 wk after toxic exposure. In the second study, effects from five dosage levels were measured at 1, 4, and 10 wk, and the highest dosage level was evaluated over 44 wk. TEM produced an expected dose related loss of spermatogenic activity and subsequent recovery as determined by dual-parameter (DNA, RNA) flow cytometry (FCM) measurements of testicular cells.