Record Display for the EPA National Library Catalog


Main Title Comparison of Cytopathogenicity, Immunofluorescence and In situ DNA Hybridization as Methods for the Detection of Adenoviruses.
Author Hurst, C. J. ; McClellan, K. A. ; Benton., W. H. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. Toxicology and Microbiology Div.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/548;
Stock Number PB91-116053
Additional Subjects Deoxyribonucleic acids ; Adenoviruses ; Sewage ; Reprints ; Viral cytopathogenic effect ; Fluorescent antibody technic ; Nucleic acid hybridization ; Cell line ; Plaque assay
Library Call Number Additional Info Location Last
NTIS  PB91-116053 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Three different methods were compared for their efficiency at detection of adenoviruses. The samples examined for viral analysis consisted of concentrates prepared from raw sewage, chosen as being representative of the spectrum of viruses being intestinally shed from a large population at any given time. When using one single cell line, HEp-2, the overall numbers of adenoviruses detected using cytopathogenicity and immunofluorescence were roughly equal. In-situ hybridization was approximately forty percent more sensitive than eital. In-situ hybridization was approximately forty percent more sensitive than either of these other methods as determined by average virus titers for the different samples, and also proved to be better by means of a nonparametric comparison. The 293 cell line was approximately five times more sensitive for detecting adenoviruses by cytopathogenicity as compared with the HEp-2 cell line, but proved unsuitable in our hands for quantitatively detecting indigenous adenoviruses by immunofluorescence. The relative number of indigenous adenoviruses present in the sewage concentrates we examined was, on average, ninty-four fold greater than that of enteroviruses. Assay of enteroviruses was performed by plaque assay in the BGM cell line.