Record Display for the EPA National Library Catalog


Main Title Firefly luciferase ATP assay development for monitoring bacterial concentrations in water supplies /
Author Picciolo, Grace L. ; Chappelle, Emmett W. ; Deming, Jody W. ; Thomas, Richard R. ; Nibley., David A.
Other Authors
Author Title of a Work
Picciolo, Grace Lee.
CORP Author National Aeronautics and Space Administration, Greenbelt, MD. Goddard Space Flight Center.;Municipal Environmental Research Lab., Cincinnati, OH.
Publisher U.S. Environmental Protection Agency, Municipal Environmental Research Laboratory,
Year Published 1981
Report Number EPA/600/2-81/014
Stock Number PB81-163271
Subjects Drinking water--Contamination--Measurement ; Bacteria ; Adenosine triphosphate ; Luciferase
Additional Subjects Water supply ; Enzymes ; Bioassay ; Development ; Concentration(Composition) ; Bacteria ; Automation ; Potable water ; Microorganisms ; Sensitivity ; Quality control ; Filtration ; Adenosine triphosphate ; Luciferase ; Monitoring ; Water pollution detection
Library Call Number Additional Info Location Last
NTIS  PB81-163271 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 37 pages : illustrations ; 28 cm
This research program was initiated to develop a rapid, automatable system for measuring total viable microorganisms in potable drinking water supplies using the firefly luciferase ATP assay. The assay was adapted to an automatable flow system that provided comparable sensitivity with established methodology 100,000 bacteria per ml) and assays in less than 2 minutes. Quality controls for required reagents were established. To achieve the sensitivity necessary for bacterial measurements in water, the sample must be concentrated prior to assay. Filtration systems were evaluated for ability to concentrate bacteria from large sample volumes rapidly, efficiently, and without damage to the organisms. Results indicated a limited capability of most systems to meet project criteria. Promising results (200 to 600-fold concentration and up to 88% recovery of bacteria) were obtained using hollow fiber concentration systems modified to incorporate repeated backwash steps. Due to manpower constraints, Goddard Space Flight Center had to discontinue projects not directly related to the space mission and was unable to continue evaluation and development of a satisfactory concentration system to go with the flow system for the ATP assay.
Cover title. "February 1981." "EPA-600/2-81-014." Microfiche;