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Main Title Triphenyl Phosphite-Induced Ultrastructural Changes in Bovine Adrenomedullary Chromaffin Cells.
Author Anderson, J. K. ; Veronesi, B. ; Jones, K. ; Lapadula, D. M. ; Abou-Donia, M. B. ;
CORP Author Duke Univ. Medical Center, Durham, NC.;Environmental Protection Agency, Research Triangle Park, NC. Neurotoxicology Div.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/271;
Stock Number PB92-209337
Additional Subjects Adrenal medulla ; Chromaffin granules ; Toxicology ; Cultured cells ; Cattle ; Electron microscopy ; Paraoxon ; Isoflurophate ; Nervous system ; Adenosine ; Adenosine triphosphate ; Reprints ; Triphenyl phosphite
Library Call Number Additional Info Location Last
NTIS  PB92-209337 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Primary cultures of bovine adrenomedullary chromaffin cells were treated with the phosphorous acid ester, triphenyl phosphite (TPP), and the morphological changes assessed by transmission electron and scanning microscopy. Parallel studies were carried out with the cholinergic compound 0,0-diethyl-4 nitrophenyl phosphate (paraoxon) and the delayed neurotoxicant 0,0-diisopropylphosphorofluoridate (DFP). Transmission and scanning electron microscopy revealed that treatment with both of these organophosphorous compounds did not produce the ultrastructural effects that were seen with TPP. Treatment with 100 micromoles TPP for 4 or 24 hours caused a marked inhibition (90% relative to controls) of adenosine incorporation. Neither 100 micromoles paraoxon or 100 micromoles DFP had an inhibitory effect on incorporation. The results support a specific affect of the triphenylphosphite, TPP, not a general toxic effect of organophosphorous compounds since the cholinergic agent paraoxon and the delayed neurotoxic compound DFP did not alter the cells ultrastructure.