Record Display for the EPA National Library Catalog


Main Title Predicting Biotransformations in the Subsurface: Relationship between the ATP (Adenosine triphosphate) Content of Subsurface Material and the Capacity of Subsurface Organisms to Degrade Toluene.
Author Wilson, J. ; Miller, G. ; Ghiorse, W. C. ; Leach, F. ;
CORP Author Robert S. Kerr Environmental Research Lab., Ada, OK. ;Oklahoma Univ., Norman. ;Cornell Univ., Ithaca, NY. ;Oklahoma State Univ., Stillwater.
Year Published 1985
Report Number EPA/600/D-85/028;
Stock Number PB85-161560
Additional Subjects Microorganisms ; Water pollution ; Ground water ; Hazardous materials ; Industrial wastes ; Petroleum products ; Toluene ; Samples ; Biodeterioration ; Hydrocarbons ; Metabolism ; Solid waste disposal ; Earth fills ; Biological indicators ; Chemical spills ; Adenosine triphosphate ; Liquid waste disposal ; Biotransformation
Library Call Number Additional Info Location Last
NTIS  PB85-161560 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Deeper subsurface material was collected in a manner that prevented contamination by surface microorganisms. This material was analyzed for ATP content, and for its capacity to degrade toluene, a common organic contaminant of ground water originating from release of petroleum products. In material from a pristine site near Lula, OK where the permanent water table was at 4m the ATP content of material from 1.5, 2.9, and 4.6m was 1.2, 1.1, and 0.14 micrograms/g respectively. Material from all three depths degraded toluene rapidly. Material from just below the water table at 7.5m at a near-pristine site in Conroe, TX, had an ATP content of only 0.017 mg/g, and could not degrade toluene. All four samples had essentially the same number of cells as determined by Acridine Orange Direct Count. Studies are in progress to determine whether deeper material from the Lula site with a low ATP content (0.03 micrograms/g) can degrade toluene in order to determine if ATP is a candidate index-compound to predict active biotransformation of industrial contaminants.