| Main Title |
The role of T cells in the regulation of acrolein-induced pulmonary inflammation and epithelial-cell pathology / |
| Other Authors |
|
| Publisher |
Health Effects Institute, |
| Year Published |
2009 |
| OCLC Number |
555687580 |
| Subjects |
T cells ;
Pneumonia ;
Epithelial cells ;
Acrolein ;
Pneumonia ;
Epithelial Cells--pathology ;
Acrolein ;
T-Lymphocytes
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EKBD |
HEI-146 |
|
Research Triangle Park Library/RTP, NC |
03/29/2010 |
|
| Collation |
vii, 42 p. : ill. ; 28 cm. |
| Notes |
"December 2009." Includes bibliographical references. |
| Contents Notes |
Methods. Mice -- Acrolein exposure -- Bronchoalveolar lavage -- Cell and differential counts -- Tissue fixation, histology, and immunohistochemistry -- Quantification of mucous-cell metaplasia -- BAL mucin assay -- Lung-cell isolation and flow cytometry -- T-cell purification -- RNA isolation and amplification -- Experimental design of microarrays -- Microarray hybridization -- Microarray analysis -- Quantitative real-time PCR -- ELISA -- Statistical analysis -- Results. Acrolein-induced pulmonary pathology -- Injury to BAL epithelial cells -- Apoptosis of pulmonary epithelial cells -- Pulmonary inflammation -- Levels of macrophage mediators in the lung -- Purification of pulmonary lymphocytes -- Microarray data -- Cytokines and chemokines -- Discussion and conclusions. Effects of acrolein on pulmonary pathology -- Roles of [alpha][beta] T cells and [gamma][delta] T cells -- Transcriptional analysis of [alpha][beta] T cells and [gamma][delta] T cells. |
