Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title The role of T cells in the regulation of acrolein-induced pulmonary inflammation and epithelial-cell pathology /
Other Authors
Author Title of a Work
Borchers, Michael T.
Publisher Health Effects Institute,
Year Published 2009
OCLC Number 555687580
Subjects T cells ; Pneumonia. ; Epithelial cells ; Acrolein. ; Pneumonia. ; Epithelial Cells--pathology. ; Acrolein. ; T-Lymphocytes.
Library Call Number Additional Info Location Last
EKBD  HEI-146 Research Triangle Park Library/RTP, NC 03/29/2010
Collation vii, 42 p. : ill. ; 28 cm.
"December 2009." Includes bibliographical references.
Contents Notes
Methods. Mice -- Acrolein exposure -- Bronchoalveolar lavage -- Cell and differential counts -- Tissue fixation, histology, and immunohistochemistry -- Quantification of mucous-cell metaplasia -- BAL mucin assay -- Lung-cell isolation and flow cytometry -- T-cell purification -- RNA isolation and amplification -- Experimental design of microarrays -- Microarray hybridization -- Microarray analysis -- Quantitative real-time PCR -- ELISA -- Statistical analysis -- Results. Acrolein-induced pulmonary pathology -- Injury to BAL epithelial cells -- Apoptosis of pulmonary epithelial cells -- Pulmonary inflammation -- Levels of macrophage mediators in the lung -- Purification of pulmonary lymphocytes -- Microarray data -- Cytokines and chemokines -- Discussion and conclusions. Effects of acrolein on pulmonary pathology -- Roles of [alpha][beta] T cells and [gamma][delta] T cells -- Transcriptional analysis of [alpha][beta] T cells and [gamma][delta] T cells.