Record Display for the EPA National Library Catalog


Main Title Comparison of Mutagenicity Results for Nine Compounds Evaluated at the 'hgprt' Locus in the Standard and Suspension CHO Assays.
Author Moore, M. M. ; Parker, L. ; Huston, J. ; Harrington-Brock, K. ; Dearfield, K. L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/019;
Stock Number PB91-183475
Additional Subjects Mutagens ; Chromosome mapping ; Hypoxanthine phosphoribosyltransferase ; Mutagenicity tests ; Chinese hamsters ; Comparative evaluations ; Acrylates ; Ethyl methanesulfonate ; Methyl methanesulfonate ; Acridines ; Tables(Data) ; Dose-response relationships ; Thymidine kinase ; Reprints ; Mouse lymphoma assay
Library Call Number Additional Info Location Last
NTIS  PB91-183475 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The Chinese hamster ovary (CHO) assay, which measures newly induced mutations at the hypoxanthine-guanine phosphoribosyltransferase (hgprt) locus, has been widely used for mutagenesis testing. The insensitivity of the standard assay to some genotoxic agents has been speculated to be due to the relatively small number of cells used in the assay. The present study compares the standard monolayer assay with a suspension adapted assay which uses cell numbers comparable to that of the L5178Y mouse lymphoma assay. Nine compounds, ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), 2-methoxy-6-chloro-9-(3-(ethyl-2-chloroethyl)-aminopropylamino)-acridine 2HCl (ICR 170), methyl acrylate, ethyl acrylate, tetraethylene glycol diacrylate, trimethylolpropane triacrylate, 2-ethylhexyl acrylate, and dicyclopentenyloxyethyl methacrylate were evaluated in the monolayer and suspension assays. Both assays gave the same overall positive/negative evaluation for the test compounds. There were some quantitative differences in the mutant frequency for the three compounds found to be mutagenic (EMS, MMS, and ICR 170). The acrylates (many of which appear to exert their genotoxic effect through a clastogenic mechanism) were negative in both test systems. The use of the suspension assay did not improve the ability of the hgprt locus to detect the genotoxicity of the acrylates.