Record Display for the EPA National Library Catalog


Main Title Flow Cytometry of Acridine Orange Stained Sperm Is a Rapid and Practical Method for Monitoring Occupational Exposure to Genotoxicants.
Author Evenson, D. P. ;
CORP Author South Dakota State Univ., Brookings.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/D-85/234;
Stock Number PB86-116407
Additional Subjects Toxicology ; Reproduction(Biology) ; Fertility ; Acridine orange ; Flow cytometry ; Toxic substances ; Monitoring
Library Call Number Additional Info Location Last
NTIS  PB86-116407 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Public awareness is growing concerning the reproductive consequences of the numerous environmental and occupational chemicals. Exposure of germ cells within the seminiferous tubules of the mammalian testis to chemical toxins often causes severe perturbation of cell growth, division and differentiation. Quantitative and qualitative reduction of sperm may have adverse consequences for fertility, and normality of fetus. To detect toxic effects of chemical exposure on germ cells, it is of prime importance to develop more sensitive and practical methods by which putative alterations in germ cells may be investigated. Previously selected (Overstreet 1984) criteria for male reproductive risk assessment required the tests be (a) objective, (b) technically sound, (c) biologically stable, (d) sensitive, and (e) feasible. Flow cytometric measurement of acridine orange stained sperm meets all of these criteria for monitoring occupational exposure to genotoxicants. This conclusion is based on two independent studies of sperm obtained from: (1) toxin exposed mice, and (2) patients attending infertility and cancer treatment clinics.