| Main Title |
V(D)J Recombinase-Mediated Deletion of the 'hprt' Gene in T-Lymphocytes from Adult Humans. |
| Author |
Fuscoe, J. C. ;
Zimmerman, L. J. ;
Harrington-Brock, K. ;
Burnette, L. ;
Moore, M. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Vermont Univ., Burlington. Genetics Lab. |
| Publisher |
c1992 |
| Year Published |
1992 |
| Report Number |
EPA/600/J-92/422; |
| Stock Number |
PB93-141216 |
| Additional Subjects |
Hypoxanthine phosphoribosyltransferase ;
T-lymphocyte gene rearrangement ;
Mutagenesis ;
Chromosome deletion ;
Base sequence ;
DNA mutational analysis ;
Mutations ;
Gene expression ;
Exons ;
Lymphocytes ;
Polymerase chain reaction ;
Southern blotting ;
Adults ;
Clone cells ;
Newborns ;
Reprints ;
V(D)J recombinase
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-141216 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
The hprt T-cell cloning assay allows the detection of mutations occurring in vivo in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene of T-lymphocytes. In the report, we examined a collection of 314 hprt-deficient clones derived from adult humans for evidence that the mutations were caused by the illegitimate activity of V(D)J recombinase by analyzing exons 2+3 deletion mutations. DNA sequence analysis of deletion breakpoint junctions showed that eight of the mutations were the result of V(D)J recombinase activity. The frequency of the recombinase-mediated mutations was similar in the adults and newborns (2-4 x 10 to the power of -7). Unregulated expression of V(D)J recombinase activity may be an important mechanism for genomic rearrangements in the genesis of cancer. |
