Record Display for the EPA National Library Catalog


Main Title Characterization of the DNA of a Nonoccluded Baculovirus, Hz-1V.
Author Huang, Yuan-Shen ; Hedberg, M. ; Kawanishi, C. Y. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-600/J-82-113;
Stock Number PB83-213926
Additional Subjects Deoxyribonucleic acids ; Viruses ; Cells(Biology) ; Purification ; Isolation ; Electron microscopy ; Reprints ; Baculoviruses ; Cell lines ; Endonucleases
Library Call Number Additional Info Location Last
NTIS  PB83-213926 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The DNA of the nonoccluded baculovirus (Hz-1V) obtained from the IMC-Hz-1 cell line was characterized by physicochemical and restriction endonuclease techniques. Hz-1V DNA isolated from purified virus had buoyant densities of 1.58 and 1.54 g/ml in CsC1-ethidium bromide density gradients, which corresponded to supercoiled and to relaxed circular and linear DNA, respectively. Neutral CsC1 equilbrium centrifugation indicated that the Hz-1V DNA had a buoyant density of 1.7024 g/m1, which corresponded to a guanine-plus-cytosine (G + C) content of 43%. Thermal denaturation indicated a high G t C domain (s) in the Hz-1V genomic DNA. The domain (s), which included about 11% of the total genomic DNA, exhibited a Tms corresponded to G t C contents of 42 and 67%, respectively.