Record Display for the EPA National Library Catalog


Main Title Cell Culture Studies with the IMC-Hz-1 Nonoccluded Virus.
Author Ralston, Ann L. ; Huang, Yuan-Shen ; Kawanishi, C. Y. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-600/J-81-168;
Stock Number PB83-231225
Additional Subjects Cell cultures ; Viruses ; Reprints ; Cell lines ; Adventitious agent
Library Call Number Additional Info Location Last
NTIS  PB83-231225 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
Studies were conducted on an adventitious agent (Hz-lv) isolated from the IMC-Hz-1 cell line. It appeared identical to the virus first obtained by Granados et al. from a persistent infection of this cell line. Restriction endonuclease digestion of Hz-lv DNA indicated the agent was different from the S nuclear polyhedrosis virus (HzSNPV) of the host species, Heliothis zea, from which the IMC-Hz-1 cell line was derived by Hink and Ignoffo (W.F. Hink and C.M. Ignoffo, 1970, Exp. Cell Res. 60, 307-309). Hz-1V caused extensive cytopathic effects (CPE) in cultures of TN-368 cells and was moderately infectious to several other insect cell lines. The lytic nature of the infection permitted development of a plaque assay employing TN-368 cells.