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Main Title Comparison of Mixed Flask Culture and Standardized Laboratory Model Ecosystems for Toxicity Testing.
Author Shannon, L. J. ; Harrass, M. C. ; Yount, J. D. ; Walbridge, C. T. ;
CORP Author Environmental Research Lab.-Duluth, MN. ;Minnesota Univ.-Duluth. Dept. of Biology. ;Food and Drug Administration, Washington, DC.
Year Published 1986
Report Number EPA/600/D-85/214-F;
Stock Number PB87-167300
Additional Subjects Toxicity ; Copper sulfates ; Sensitivity ; Reproducibility ; Evaluation ; Costs ; Reprints ; Microcosms ; SAM(Standardized Aquatic Microcosm) method ; MFC(Mixed Flask Culture) method ; Ecosystems
Library Call Number Additional Info Location Last
NTIS  PB87-167300 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 26p
Two microecosystem protocols, the Standardized Aquatic Microcosm (SAM) method (1982) and the Mixed Flask Culture (MFC) method (1981), were compared on the basis of their response to copper sulfate. These protocols differed in microcosm structure, age, and the variables monitored. Although responses were similar with both systems, the SAM procedure provided considerably more insight into the changes in population densities and nutrient cycling responsible for the observed ecosystem level changes. The SAM protocol was more repeatable and provided a much more complete picture of toxicant effects, but was extremely labor intensive. The MFC protocol lacked repeatability and provided no information to aid in interpreting ecosystem level effects, but was capable of displaying sensitivity equal to that of the SAM procedure and required a comparatively small time investment. (Copyright (c) 1987, American Society for Testing and Materials.)