Record Display for the EPA National Library Catalog


Main Title Effects of holding time, storage, and the preservation of samples on sample integrity for the detection of fecal indicator bacteria by quantitative polymerase chain reaction (qPCR)-based assays /
Author Wymer, Larry, ; L. Wymer ; K. Oshima ; J. Paar ; M. Doolittle ; J. Lavender
CORP Author TechLaw, Inc., Bethesda, MD.; Environmental Protection Agency, Lexington, MA. Region 1.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory,
Year Published 2010
Report Number EPA 600-R-10-150
Stock Number PB2013-107494
OCLC Number 1099469954
Subjects Water--Pollution--Research--United States
Additional Subjects Bacteria ; Water pollution monitoring ; Fecal pollution ; Reagents ; Membrane filters ; Standards ; Equipment ; Supplies ; Safety ; Sampling ; Quality control ; Calibration ; Enteric pathogens ; Pollution prevention ; Waste management ; RNA gene sequences ; Quantitative polymerase chain reaction (qPCR) ; TaqMan ; Enterococci
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
ESAD  EPA 600-R-10-150 Region 10 Library/Seattle,WA 05/08/2019
NTIS  PB2013-107494 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 1 volume (various pagings) : tables, figures ;
The purpose of this project was to answer questions related to storage of samples to be analyzed by the quantitative polymerase chain reaction (qPCR)-based assays for fecal indicator bacteria. The report is divided into two parts. The first part describes studies that were performed to determine if filters that are used to collect fecal indicator bacteria can be stored frozen and analyzed at a later date. These studies were primarily directed at a specific, targeted question: can qPCR results from freezer archived samples be used to establish valid relationships between fecal indicator densities and health effects data collected from previous epidemiological studies. The second part describes studies that were performed to determine if refrigerated water samples can be held for 24 to 48 hours prior to analysis by qPCR. These studies addressed a question that may be relevant to the implementation of the qPCR method for water quality monitoring at remote locations where immediate analysis of the samples is not possible.
"EPA/600/R-10/150." "December 2010." Includes bibliographical references.