Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Interim procedures for conducting the Salmonella/microsomal mutagenicity assay (Ames test) /
Author Williams, Llewellen R. ; Preston, J. E.
Other Authors
Author Title of a Work
Preston, John E.
CORP Author Environmental Monitoring Systems Laboratory (Las Vegas, Nev.)
Publisher U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory ; Center for Environmental Research Information [distributor],
Year Published 1983
Report Number EPA-600/4-82-068
Stock Number PB88-205380
OCLC Number 10218705
Subjects Mutagenicity testing.
Additional Subjects Mutagenicity testing ; Mutagens ; Carcinogens ; Hazardous materials ; Bioassay ; Tests ; Salmonella ; In vivo analysis ; Enzymes ; Sensitivity ; Culture media ; Counting methods ; Histidine ; Separation ; Chemicals ; Ames test
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EHAM  EPA-600/4-82-068 Region 1 Library/Boston,MA 05/25/2016
EJBD ARCHIVE EPA/600-4-82-068 Headquarters Library/Washington,DC 03/28/2016
EJED  EPA 600/4-82/068 OCSPP Chemical Library/Washington,DC 12/17/2004
EKBD  EPA 600/4-82-068 Research Triangle Park Library/RTP, NC 12/04/2018
EKCD  EPA-600/4-82-068 CEMM/GEMMD Library/Gulf Breeze,FL 07/25/2018
EMBD  PB88-205380 MF NRMRL/GWERD Library/Ada,OK 12/24/1992
ESAD  EPA 600-4-82-068 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB88-205380 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/01/1988
Collation 1 volume (various pagings) : 1 form ; 28 cm
The Ames assay is a rapid-screening procedure for determining the presence of mutagenic and carcinogenic pure chemicals, complex environmental mixtures, and commercial products. The procedure described incorporates modifications designed to make Ames testing data more precise and reproducible. It is intended as an interim procedure for use in the Agency's hazardous waste monitoring program. The assay uses five standard strains of histidine-requiring Salmonella typhimurium mutants that revert after interaction with mutagens to histidine-independence (his+). Certain mutagens are directly active in the system while others require activation by mammalian enzymes added to the test system. The enzymes perform metabolic conversions mimicking those of mammalian organs in vivo. Test sensitivity to some mutagens is enhanced by additional mutations in the test strains and by the plasmid pKM101 in two of the strains. Counts of revertant colonies on test plates are compared to counts of the same strain on control plates.
Distributed to depository libraries in microfiche. "Mar. 1983." Includes bibliographical references (pages R-1-R-2). "EPA-600/4-82-068."