||Comparative Yields of Mutagens from Cigarette Smokers' Urine Obtained by Using Solid-Phase Extraction Techniques.
Williams, R. W. ;
Pasley, T. ;
Watts, R. ;
Inmon, J. ;
Fitzgerald, J. ;
||Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Chemical analysis ;
Salmonella typhimurium ;
Ultraviolet spectroscopy ;
High pressure liquid chromatography ;
Mutagenicity tests ;
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Urine from cigarette smokers was prepared for mutagenicity testing by extracting mutagens with solid-phase extraction columns. Commercially available prepacked bonded silicas (Octadecycle, cyclohexyl, cyanopropyl) were compared for their ability to concentrate the urinary mutagens. Recovered urinary metabolites were evaluated for mutagenic activity by using a microreversion assay with Salmonella typhimurium. Dose-response data indicated that while mutagens were recovered by all three adsorbents, samples prepared by using the bonded cyanopropyl columns yielded the most bioactivity and/or the least amount of test organism toxicity. (Copyright (c) 1989 Alan R. Liss, Inc.)