Record Display for the EPA National Library Catalog


Main Title Comparative Yields of Mutagens from Cigarette Smokers' Urine Obtained by Using Solid-Phase Extraction Techniques.
Author Williams, R. W. ; Pasley, T. ; Watts, R. ; Inmon, J. ; Fitzgerald, J. ;
CORP Author Environmental Health Research and Testing, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA-68-02-4456; EPA/600/J-89/223;
Stock Number PB90-146077
Additional Subjects Mutagens ; Smoking ; Chemical analysis ; Urine ; Graphs(Charts) ; Bioassay ; Salmonella typhimurium ; Ultraviolet spectroscopy ; Reprints ; High pressure liquid chromatography ; Mutagenicity tests ; Dose-response relationships
Library Call Number Additional Info Location Last
NTIS  PB90-146077 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Urine from cigarette smokers was prepared for mutagenicity testing by extracting mutagens with solid-phase extraction columns. Commercially available prepacked bonded silicas (Octadecycle, cyclohexyl, cyanopropyl) were compared for their ability to concentrate the urinary mutagens. Recovered urinary metabolites were evaluated for mutagenic activity by using a microreversion assay with Salmonella typhimurium. Dose-response data indicated that while mutagens were recovered by all three adsorbents, samples prepared by using the bonded cyanopropyl columns yielded the most bioactivity and/or the least amount of test organism toxicity. (Copyright (c) 1989 Alan R. Liss, Inc.)