Record Display for the EPA National Library Catalog


Main Title Effect of Phenobarbital on Diethylnitrosamine and Dimethylnitrosamine Induced Hepatocellular Tumors in Male B6C3F1 Mice.
Author Klaunig, J. E. ; Weghorst, C. M. ; Pereira, M. A. ;
CORP Author Health Effects Research Lab., Cincinnati, OH. ;Medical Coll. of Ohio at Toledo.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/381;
Stock Number PB90-103169
Additional Subjects Toxicity ; Liver neoplasms ; Phenobarbital ; Carcinogens ; Mice ; Males ; Body weight ; Reprints ; Diethylnitrosamine ; Dimethylnitrosamine ; Dose-response relationships ; Drinking water ; Organ weight
Library Call Number Additional Info Location Last
NTIS  PB90-103169 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The effect of the type of carcinogen initiator on the ability of phenobarbital to promote hepatic tumor formation in 15-day-old initiated male B6C3F1 mice was evaluated. Fifteen day old male B6C3F1 mice were divided into 6 groups of 10 mice each. Groups 1 and 2 received a single intraperitoneal (ip) injection of diethylnitrosamine (DENA) (5 micro g/gbw). Groups 3 and 4 received a single ip injection of dimethylnitrosamine (DMNA) (5 micro g/gbw). Groups 5 and 6 received a single ip injection of saline. At weaning (28 days of age), mice in groups 2, 4, and 6 received phenobarbital (500 mg/ml) in their drinking water. Mice in groups 1, 3, and 5 received deionized drinking water. Drinking water treatment continued for 24 weeks at which time mice were sampled. At sampling, mice were examined for hepatic tumors by histology. Mice in Groups 5 (no treatment) and 6 (PB only) did not exhibit hepatic tumors. Groups 2 (DENA + PB) displayed a decrease in hepatic adenomas from that of Group 1 (DENA only) confirming previous observations. Treatment with DMNA and PB (Group 4) resulted in a significant increase in both hepatic adenoma incidence and number over that of DMNA only (Group 3) treated mice. (Copyright (c) 1988 Elsevier Scientific Publishers, Ireland Ltd.)