Record Display for the EPA National Library Catalog

RECORD NUMBER: 25 OF 27

OLS Field Name OLS Field Data
Main Title Use of Prolonged Treatment and the Fluctuation Test to Detect Mutations in Human Fibroblasts Treated with Methyl Methanesulfonate.
Author Huang, Shiu L. ; Biddix, Gwyn ; Waters, Michael D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-600/J-82-302;
Stock Number PB83-189563
Additional Subjects Toxicology ; Humans ; Reprints ; Methyl methanesulfonate ; Fibroblasts ; Mutagenesis ; Fluctuation test
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB83-189563 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 10p
Abstract
The methyl methanesulfonate (MMS) induction of 6-thioguanine-resistant (6TG) mutants in non-synchronized human fibroblasts has been quantitatively characterized. A 24-h MMS treated failure to induce mutations in non-synchronized cultures. However, exposure of growing cells to MMS for 3 days caused a significant increase in the mutation frequencies. The mutations induction was dose-dependent. The fluctuation test was also useful in detecting the mutagenic activity of MMS in human fibroblasts. Prolonged treatment of cultures made the human cell mutagenesis testing system more sensitive.