||Tissue-Specific Sister Chromatid Exchange Analyses in Mutagen-Carcinogen Exposed Animals.
Allen, James W. ;
Langenbach, Robert ;
Waters, Michael D. ;
Sharief, Yousuf ;
||Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Deoxyribonucleic acid ;
Laboratory animals ;
Sister chromatid exchange ;
||Some EPA libraries have a fiche copy filed under the call number shown.
The phenomenon of sister chromatid exchange (SCE) has been extensively reviewed. Sister chromatid exchanges are intrachromosomal events, wherein segments of DNA are reciprocally swapped between the chromatids. They are most easily studied with 5-bromodeoxyuridine (BrdU) dye methodology, which effectively differentiates the sister chromatids so that exchanges between them are detectable as staining discontinuities. Presumablly, the exchange sites are at homologous loci and no inequality in the amount of translocated material results. Sister chromatid exchange is not known to alter cell viability or function; its spontaneous frequency and biological importance are uncertain. Yet, early autoradiography studies in cultured cells revealed elevated SCE frequencies as an effect of mutagen and carcinogen exposures.