Record Display for the EPA National Library Catalog


Main Title Isolation of a Soluble Cadmium-Binding Protein from Pulmonary Macrophages.
Author Cox, C. Christine ; Waters, Michael D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Environmental Toxicology Div.
Year Published 1978
Report Number EPA-600/J-78-149;
Stock Number PB80-129950
Additional Subjects Protein ; Isolation ; Cadmium ; Rabbits ; Reprints ; Macrophages
Library Call Number Additional Info Location Last
NTIS  PB80-129950 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
A soluble cadmium-binding protein, with properties similar to metallothionein, has been isolated from rabbit alveolar macrophages. The macrophages were cultured in Medium 199 with Earle's salts for 24 hr in the presence of 10 micromoles CdCl2 and carrier-free 109Cd as a tracer. The isolation procedure began with application of a 100,000 g cell supernatant to a column of Sephadex G-75 Fine. The fraction containing the greatest amount of cadmium was eluted at a relative elution volume, Ve/Vo, of 1.87. A molecular weight determination performed following Sephadex chromatography indicated that the apparent molecular weight of the impure protein was approximately 11,000. The fractions containing cadmium were pooled and purification procedures were applied, including acetone fractionation, DEAE-cellulose chromatography, and polyacrylamide gel electrophoresis. DEAE-Cellulose chromatography following acetone fractionation indicated the presence of two forms of metalloprotein as has been demonstrated previously in the isolation of cadmium-thionein from liver and kidney. The two forms of metalloprotein were subjected to polyacrylamide gel electrophoresis and, although separation was incomplete, bands obtained corresponded to those typically observed in rat liver.