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Main Title Multiple DNA Adducts in Lymphocytes of Smokers and Nonsmokers Determined by 32P-Postlabeling Analysis.
Author Jahnke, G. D. ; Thompson, C. L. ; Walker, M. P. ; Gallagher, J. E. ; Lucier., G. W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;National Inst. of Environmental Health Sciences, Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/177;
Stock Number PB91-116897
Additional Subjects Toxicology ; Lymphocytes ; Smoking ; Mutagens ; Chemical analysis ; Thin layer chromatography ; In vitro analysis ; Nucleotides ; Reprints ; DNA damage ; Benzo(a)pyrene ; High pressure liquid chromatography ; Phosphorus 32
Library Call Number Additional Info Location Last
NTIS  PB91-116897 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Identification of DNA adducts in peripheral lymphocytes could serve as a means of monitoring human exposure to potential genotoxic agents. In the study, DNA from peripheral lymphocytes of smokers and nonsmokers was examined for adducts by the P1 nuclease 32p-post-labeling technique. Thin layer chromatography (TLC) maps from both groups revealed multiple DNA adducts which ranged from no adducts for one individual to six adducts for a different individual. The total DNA adduct concentrations were approximately one adduct in 10 to the seventh-10 to the eighth power normal nucleotides. Comparison of the adduct TLC profiles revealed individual variation in both pattern and level of DNA adducts. The type and amount of adduct was not influenced by smoking history and remained unchanged in four out of six subjects who were resampled after a one month interval. One adduct detected in lymphocyte DNA co-migrated on TLC with an adduct derived by in vitro incubation of lymphocytes with benzo(a)pyrene (B(a)P). The 3H-nucloside values were consistent with values obtained by 32p-postlabeling of the same sample (correlation coefficient of 0.88). No relationship was apparent between the capacity of lymphocytes to form a (3H)-B(a)P-derived adduct in vitro and the concentration of the adduct, or total adducts present in untreated lymphocytes.