Record Display for the EPA National Library Catalog


Main Title Biochemical Measures of Coral Metabolic Activity, Nutritional Status and Microbial Infection with Exposure to Oil and Gas Well Drilling Fluids.
Author White, D. C. ; Nickels, J. S. ; Gehron, M. J. ; Parker, J. H. ; Martz, R. F. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1987
Report Number EPA-R-807321; EPA/600/D-87/073;
Stock Number PB87-166955
Additional Subjects Coral ; Drilling fluids ; Water pollution ; Stress(Physiology) ; Exposure ; Sea water ; Chloroform ; Carbinols ; Fatty acids ; Metabolism ; Chromatographic analysis ; Lipids ; Amino acids ; Growth ; Biochemistry ; Nutrition ; Montastrea annularus ; Coral
Library Call Number Additional Info Location Last
NTIS  PB87-166955 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 28p
The reef building coral Montastrea annularus was exposed continuously to suspensions of oil and gas-well drilling fluids at concentrations of 0.1 ml/liter, 0.01 ml/liter, and 0.001 ml/liter in flowing seawater at the U.S. Naval Stage I platform. After 6 weeks exposure, coral fragments of 30 to 60 cm sq cm surface area were broken off, rinsed in seawater, and extracted in a one-phase chloroform-methanol seawater extract and returned to the laboratory, the lipids were analyzed for their phospholipid content, alkyl fatty acid composition, and neutral lipid triglyceride glycerol. The aqueous phase was analyzed for free amino acid composition. Biochemical evidence of stress was reflected in the cessation of growth as measured in depressed diacyl phospholipid. Detailed analysis of the acyl fatty acid composition by capillary gas chromatography showed changes in polyenoic fatty acids, suggesting possible changes in the metabolism of the fatty acids induced by the exposure to the drilling fluids.