||Use of Prolonged Treatment and the Fluctuation Test to Detect Mutations in Human Fibroblasts Treated with Methyl Methanesulfonate.
Huang, Shiu L. ;
Biddix, Gwyn ;
Waters, Michael D. ;
||Health Effects Research Lab., Research Triangle Park, NC.
Methyl methanesulfonate ;
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The methyl methanesulfonate (MMS) induction of 6-thioguanine-resistant (6TG) mutants in non-synchronized human fibroblasts has been quantitatively characterized. A 24-h MMS treated failure to induce mutations in non-synchronized cultures. However, exposure of growing cells to MMS for 3 days caused a significant increase in the mutation frequencies. The mutations induction was dose-dependent. The fluctuation test was also useful in detecting the mutagenic activity of MMS in human fibroblasts. Prolonged treatment of cultures made the human cell mutagenesis testing system more sensitive.